Mat Titers Leptospirosis

Human leptospirosis is an acute febrile illness that presents with many manifestations and is found worldwide.

Mat titers leptospirosis. The mat or microscopic agglutination test detects the presence of antibodies against leptospira in a dog s blood. An interpretation is included in each mat report at no extra charge and additional consultation is also available. Horses that abort due to leptospirosis exposure tend to have very high mat titers. A series of cultures of different leptospires are incubated with serial dilutions of serum.

Laboratory studies can confirm the diagnosis and determine the extent of organ involvement. Titers of greater than 100 are usually considered significant although these may be due to vaccination. The mat is an excellent test but it. Sera are screened at a 1 100 dilution and those showing agglutination are then serially diluted further to determine a titer endpoint.

Detection of antibodies using the microscopic agglutination test mat has been the most common diagnostic method used for the diagnosis of canine leptospirosis 10 vaccination with commercially available leptospirosis vaccines will produce detectable mat titers 10 see algorithm 2 for more information on mat results. As with other serology assays vaccination complicates the interpretation of titers. In leptospirosis antibodies can be demonstrated in the serum by mat. Major disadvantages of microscopic agglutination test mat.

Results are expressed as the reciprocal of the highest dilution where agglutination is observed. The mat is the keystone for a correct diagnosis wolff 1954. If the level of antibodies called a titer is high enough or can be shown to be rising over time then infection is confirmed. A single elevated mat titer in a patient with a compatible febrile illness and suspected exposure suggests acute leptospirosis.

Lepto mat result interpretation the mat is a qualitative and quantitative test having high diagnostic specificity and relatively low sensitivity. For the reading of mat titers it is highly recommended to use a darkfield microscope with a high contrast between the leptospires and the dark background. Testing may include culture and or serology. Titers against serovar autumnalis are available in our expanded panel test code 95066 or as an add on test code 95054 by request.

What is the mat test for leptospirosis. A severe form which includes jaundice kidney failure and bleeding is called weil disease. Does the mat always work. In equine abortion cases a mat titer of 1 6400 or higher is considered.